One-shots are cards which trash themselves, or otherwise remove themselves from your custody, when you play them. For some cards this self-trashing is a limitation to prevent you from getting multiple uses out of an otherwise powerful card; for a few, however, it's a feature to prevent the card from cluttering up your deck once you've gotten use out of it.
 List of one-shot cards
Cards in italics have been removed.
 Mandatory one-shots
These cards always remove themselves from your deck when you play them.
- Dominion: Feast
- Seaside: Embargo, Island, Treasure Map
- Dark Ages: Madman, Pillage, Spoils
- Adventures: Champion, Distant Lands, Hireling
- Nocturne: Changeling, Wish
- Renaissance: Acting Troupe, Experiment
- Promo: Prince is strictly speaking optional as a one-shot, but it has no effect if you play it but don't set it aside.
 Optional or potential one-shots
- Intrigue: Mining Village—you may trash this when you play it for an additional benefit
- Cornucopia: Horn of Plenty—this trashes itself if you use it to gain a Victory card
- Dark Ages: Death Cart—you trash this if you cannot, or decide not to, trash another Action card
- Dark Ages: Knights—a Knight trashes itself if it is used to trash another Knight
- Adventures: Travellers, such as Hero, can exchange themselves for another card when discarded from play
- Adventures: Raze can trash another card or itself for its effect
- Adventures: Wine Merchant gives you a strong bonus when you play it and costs a penalty to call it back from your Tavern mat; it can function as a one-shot if you don't want to save the to get it back
- Empires: Engineer can optionally trash itself to gain an additional card
- Empires: Encampment returns to its pile if you fail to reveal a Gold or Plunder
- Empires: Small Castle can trash another Castle or itself for its effect
- Empires: Farmers' Market only trashes itself if there are 4 or more tokens on its pile
- Nocturne: Pixie—you may trash this to receive a Boon twice
- Nocturne: Tragic Hero trashes itself if you have too many cards in hand
 Other conditional self-trashers
These cards don't self-trash when you play them, but may do so later the same turn if certain conditions are met.
- Dark Ages: Hermit—this trashes itself if you don't buy a card on the turn when you play it
- Dark Ages: Urchin—you may trash this if you play another Attack while this is in play